Sabouraud Dextrose Broth Himedia MH033-500G is recommended for the cultivation of yeasts, molds, and aciduric microorganisms from pharmaceutical products. By the microbial limit testing by the harmonized methodology of USP/EP/BP/JP. Composition complies with specifications in Chapter 62 Microbiological Examination of Nonsterile Products: Tests for Specified Microorganisms. Sabouraud Dextrose Broth 500g (Himedia) is a type of growth medium used for cultivating fungi, particularly yeasts and molds. It is a specialized formulation designed to support the growth of fungi while inhibiting bacterial growth to some extent.

Components of Sabouraud Dextrose Broth:

• Dextrose: A form of glucose that provides an energy source for fungi.
• Peptone: A source of amino acids and nitrogen, essential for fungal growth.
• Agar (if in the broth form, it will be in liquid form): This medium is typically liquid (as opposed to Sabouraud Dextrose Agar which is solid) and does not contain agar to solidify it.

Purpose:

• Culturing fungi, including yeasts and molds.
• Testing for fungal growth in clinical, environmental, or industrial samples.
• Detection of fungal infections in medical specimens.

Characteristics:

• The medium has a slightly acidic pH (usually around 5.6), which helps suppress bacterial growth while promoting fungal growth.
• It is suitable for the isolation and cultivation of a wide variety of fungi, including dermatophytes, Candida species, and other pathogens.

Instructions for Use:

1. Preparation: Dissolve the required amount of Sabouraud Dextrose Broth in distilled water (usually 30g of broth powder per liter of water).
2. Sterilization: Autoclave the medium at 121°C for 15 minutes.
3. Inoculation: Inoculate the medium with the fungal sample to be tested.
4. Incubation: Incubate at 25–30°C, and observe for fungal growth.

Packaging:

• The product you mentioned (500g) would be suitable for preparing several liters of the broth medium, depending on the amount required per liter. Click for TDS

Applications of Sabouraud Dextrose Broth:

• Clinical Microbiology: Used in the diagnostic process of fungal infections.
• Mycology Research: For studying fungal species in laboratory conditions.
• Environmental Testing: To detect the presence of fungal spores in air or water samples.