Bismuth Sulphite Agar (BSA) selectively isolates Salmonellae from feces, urine, sewage, and other materials. Bismuth Sulphite Agar (BSA) is a selective culture medium used primarily for isolating and identifying Salmonella species, particularly Salmonella typhi and Salmonella enteritidis. It contains bismuth sulfite, which inhibits the growth of many other bacteria, allowing for the selective growth of Salmonella. Brand: HiMedia, Made in India. Intended Use: Recommended for selective isolation and enumeration of Salmonella species from food samples. This medium’s composition and performance criteria are as per specifications laid down in ISO 6579-1:2017.

Ingredients in Bismuth Sulphite Agar:

• Enzymatic digest of animal tissues meat: 10.00 Gms/ltr.
• Extract: 5.00 Gms/ltr.
• Dextrose: 5.00 Gms/ltr.
• Disodium Hydrogen phosphate anhydrous: 4.00 Gms/ltr.
• Ferrous sulfate anhydrous: 0.300 Gms/ltr.
• Bismuth sulfite indicator: 8.00 Gms/ltr.
• Brilliant Green: 0.025 Gms/ltr.
• Agar: 20 Gms/Litre
• Final pH (at 25°C): 7.7±0.2 Gms/ltr.
• Made in India

Principle And Interpretation

The Salmonellae constitute the most taxonomically complex group of bacteria among Enterobacteriaceae (1). Human Salmonella infections are most commonly caused by ingestion of food, water, or milk contaminated by human or animal excreta. Humans are the only reservoirs of S.Typhi (2). Of the various media employed for the isolation and preliminary identification of Salmonellae, particularly Salmonella Typhi; Bismuth Sulphite Agar is the most productive. Bismuth Sulphite Agar is a modification of the original Wilson and Blair Medium (3-5). It is also recommended by various Associations (2,6-8) for the isolation and preliminary identification of Salmonella Typhi and other Salmonellae from pathological materials, sewage, water, food, and other products. Bismuth Sulphite Agar (M027I) is recommended for selective isolation and enumeration of Salmonella species by the ISO Committee (8). Continue Reading… 

Type of specimen

Clinical samples- feces, Food, and meat samples. milk and milk products, animal feed, animal feces, and environmental samples.

Warning and Precautions

In Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred to in individual safety data sheets.